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Promega
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Jackson Immuno
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Image Search Results
Journal: Journal of Proteome Research
Article Title: Initial Development and Validation of a Novel Extraction Method for Quantitative Mining of the Formalin-Fixed, Paraffin-Embedded Tissue Proteome for Biomarker Investigations
doi: 10.1021/pr100812d
Figure Lengend Snippet: Comparison of representative protein extraction efficiencies for FFPE normal renal tissues with Rapigest and ACN−NH 3 HCO 3 buffers using SELDI-TOF (Ciphergen). Spectral acquisition was carried out on 1 μg protein extracts spotted on NP20 chips. (a) The postextraction protein spectra and (b) the post-tryptic digestion peptide spectra for the two extraction buffers.
Article Snippet: Digests from frozen and FFPE extractions were resuspended in 5% (v/v) ACN/0.05% TFA and 1 μg of protein extract was spotted on to a
Techniques: Protein Extraction